质粒名称: | pcDNA3/HA-FLAG RBP2 H483A |
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出品公司: | Addgene |
目录编号: | 14801 |
存储实验室: | William Kaelin |
载体骨架基本信息 |
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载体名称: | pcDNA3 |
载体出品公司: | -- |
载体抗性: | 氨苄青霉素 |
空载体大小: | 5446 bp |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 哺乳细胞表达 |
筛选标记: | 新霉素(G418) |
高拷贝/低拷贝: | 低拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | Retinoblastoma Binding Protein 2,RBP2,JARID1A,KDM5A |
物种来源: | H. sapiens (human) |
插入大小: | 4900 bp |
基因突变: | Changed His 483 to Ala, R46W |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: |
HA(骨干网上的N个终端)
标志(主干上的N个终端)
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克隆方法: | 限制性内切酶 |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | pCAAT |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pcDNA3/HA-FLAG RBP2 H483A was a gift from William Kaelin (Addgene plasmid # 14801 ; http://n2t.net/addgene:14801 ; RRID:Addgene_14801) |
参考文献部分: | The retinoblastoma binding protein RBP2 is an H3K4 demethylase. Klose RJ, Yan Q, Tothova Z, Yamane K, Erdjument-Bromage H, Tempst P, Gilliland DG, Zhang Y, Kaelin WG. Cell. 2007 Mar 9. 128(5):889-900. 10.1016/j.cell.2007.02.013 PubMed 17320163 |
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载体基本信息
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载体序列
LOCUS pcDNA3/HA-FLAG RBP2 H483A 11773 bp ds-DNA circular SYN 29-AUG-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pcDNA3/HA-FLAG RBP2 H483A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11773) AUTHORS Klose RJ, Yan Q, Tothova Z, Yamane K, Erdjument-Bromage H, Tempst P, Gilliland DG, Zhang Y, Kaelin WG TITLE The retinoblastoma binding protein RBP2 is an H3K4 demethylase. JOURNAL Cell. 2007 Mar 9. 128(5):889-900. PUBMED 17320163 REFERENCE 2 (bases 1 to 11773) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 29, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11773 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 907..933 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 952..975 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" promoter complement(7326..7344) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(7327..7344) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(7364..7381) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7370..7594 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 7640..8068 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7727..7746) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7937..7958 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(8077..8097) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 8082..8411 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 8262..8397 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8324..8343 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 8478..9272 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(8532..8551) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 9142..9161 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 9446..9567 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9483..9502) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9537..9556 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(9616..9632) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9616..9632) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9629..9651) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9640..9656 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9664..9694) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9709..9730 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9847..9864) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10018..10606) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10098..10117) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10777..11637) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11400..11419 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(11638..11742) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 901 atggcgtacc catacgatgt tccagattac gccaggatcc atcgaggagg agactacaag 961 gacgacgatg acaagggagg acgatgtcga cgaattctgc agatggggat ccgtcgacgg 1021 atggcgggcg tggggccggg gggctacgcg gcggagttcg tgccaccgcc agagtgcccc 1081 gtctttgagc cgagttggga ggagttcaca gatccgctca gctttatcgg ccgcatctgg 1141 cctttggcgg agaaaaccgg catctgcaaa attcggccgc ccaaggactg gcagcctcca 1201 tttgcctgtg aagtaaaaag ctttcgtttc actccaagag tccagcgcct gaatgaactt 1261 gaggcaatga ccagagtgag attggatttc ttggatcaac tagcaaaatt ttgggaactt 1321 caaggatcta ctctgaagat ccctgtggta gagagaaaaa tcctggatct gtatgctttg 1381 agcaagattg ttgccagcaa aggaggtttt gaaatggtca ccaaagagaa gaaatggtct 1441 aaagtgggta gtcgcttggg atatctgcca ggaaaaggaa ctgggtctct tttgaagtca 1501 cattatgaaa gaattctcta cccatatgag cttttccagt ctggtgtgag ccttatgggt 1561 gtgcagatgc ctaatttaga tcttaaagaa aaagtggagc ctgaggttct tagcactgat 1621 acccaaactt ccccagagcc aggcacaagg atgaacattc tgccgaagag aacaagacgt 1681 gtgaagactc agtcagaatc tggagatgtg agtagaaaca cggaactgaa gaaacttcag 1741 atttttgggg ctgggcccaa ggttgtgggc ttggcaatgg gaacaaaaga taaagaagat 1801 gaggtcaccc gaagacgaaa agttaccaac aggtcagacg catttaacat gcaaatgaga 1861 caacggaaag gcactctctc tgttaacttt gttgatctct atgtttgtat gttttgtggt 1921 cggggaaaca atgaagataa attgcttttg tgtgatggat gtgatgacag ctatcataca 1981 ttttgtctaa ttcctccact acctgatgtg cccaaaggag actggaggtg tcctaaatgt 2041 gtcgccgagg aatgtagcaa acctcgagaa gcctttggat ttgaacaagc tgtacgagag 2101 tatacacttc agagctttgg agagatggca gataatttta agtctgatta ttttaatatg 2161 ccagtccata tggttcccac agaactagta gaaaaggaat tttggcggct ggtaagcagc 2221 attgaagaag atgttattgt ggaatatgga gcagatatct cctcaaaaga ctttggaagt 2281 ggatttccgg tgaaggatgg gcggagaaag attctgccag aagaagagga atatgcactt 2341 tctggttgga atttgaataa catgcctgtc ctggaacagt ctgttcttgc acatattaat 2401 gtggacatct ctggtatgaa agtgccgtgg ctctatgtgg gaatgtgctt ctcttctttt 2461 tgctgggcca ttgaggatca ctggagttat tccatcaact acttgcactg gggggagcca 2521 aagacatggt atggtgtgcc atctcatgct gcagagcaac tggaggaggt gatgagagag 2581 ctggcccccg agttatttga atcccagcct gatcttctgc atcagttagt taccatcatg 2641 aaccccaacg tgctaatgga gcatggtgtg cctgtgtaca ggaccaatca gtgtgctggc 2701 gagtttgttg tgacatttcc tcgtgcctat cactctggat ttaaccaggg ctacaacttt 2761 gctgaagctg tgaacttctg tactgctgac tggttgccca ttggacgtca atgtgtaaat 2821 cattaccgac gcctaaggcg ccactgtgtc ttttcacacg aggaactaat tttcaagatg 2881 gcagcagatc cagaatgctt agatgtgggg ctggctgcca tggtctgcaa agaattgact 2941 ctcatgactg aagaagaaac acgattaaga gagtctgttg tacagatggg tgtcctgatg 3001 tcagaagaag aagtgtttga acttgttcct gatgatgagc ggcagtgttc agcatgcaga 3061 accacatgtt ttctctctgc tctcacatgt tcctgtaatc ctgagcggct tgtatgtctc 3121 taccatccaa ctgatctgtg cccctgcccc atgcagaaga aatgtcttag atatcgctac 3181 ccattagaag acctcccttc tctgctatat ggtgtaaaag tcagggcaca gtcctatgac 3241 acttgggtca gtcgtgttac agaagcattg tctgctaact tcaaccacaa aaaagatttg 3301 attgaattgc gagtaatgct ggaagatgct gaggatagga aatacccaga gaatgatctc 3361 tttcgaaaac tcagggatgc tgtaaaagaa gctgagacct gtgcttctgt ggctcagctg 3421 cttctgagca aaaagcagaa acacagacag agcccagata gtgggaggac tcggaccaaa 3481 ctgacagtgg aagaattgaa ggcctttgtc caacaacttt ttagtcttcc gtgtgtcatc 3541 agccaagctc ggcaagtaaa gaatctgcta gatgatgtgg aagagtttca tgaacgtgct 3601 caggaggcca tgatggatga aaccccagat tcttccaaac tccagatgtt gatagatatg 3661 ggctctagtc tctatgtgga actccctgaa ttaccacgac tgaagcaaga gctacaacag 3721 gctcggtggt tggacgaagt aagactgacc ttatcagatc cgcaacaagt cactttggat 3781 gtcatgaaga agctgataga ctctggggta gggttggcac cccaccatgc tgtggagaaa 3841 gcaatggctg aactacagga gctccttaca gtctctgaac gatgggaaga aaaggctaag 3901 gtctgcctac aggcaagacc gaggcacagt gtggcaagtt tagaaagcat tgtgaatgaa 3961 gccaagaaca ttccagcctt tctacccaat gtgttgtcct tgaaagaagc cttacaaaag 4021 gctcgagaat ggaccgctaa agtggaagct attcagagtg gcagcaatta cgcttatttg 4081 gagcagcttg agagcttgtc tgcgaaagga cgccctattc ctgtgcgtct tgaagcactg 4141 ccgcaagtgg aatcacaggt agcagcagca cgggcatgga gagaacggac tgggcggacg 4201 tttcttaaga agaattctag ccatacattg ttacaggtgc tgagcccccg gaccgacatt 4261 ggtgtatatg ggagtggcaa aaataggagg aaaaaagtaa aagaactaat agaaaaagaa 4321 aaagaaaagg atctggacct ggagcctctg agtgatctgg aggaaggatt ggaggaaacc 4381 agagatacag ccatggtggt ggcagttttc aaagaacggg agcaaaaaga gattgaagcc 4441 atgcattctc tcagagcagc caacctagcc aagatgacaa tggtggaccg catagaagaa 4501 gtaaaatttt gcatttgccg caagacagcc agtgggttta tgctacagtg tgagctctgc 4561 aaagactggt tccataacag ctgtgttcct cttcctaaat caagttccca aaaaaaagga 4621 tccagctggc aagctaaaga agtaaaattc ctttgccctc tttgtatgcg gtctcgaagg 4681 cccaggctag agactattct gtcactcctg gtatcccttc agaagttgcc cgtacggttg 4741 cctgaaggag aggccctgca gtgtttgaca gaacgtgcta tgagttggca agatagagcg 4801 cggcaggctc tagccacaga tgaactatcc tctgccctgg ccaaactatc tgtgttgagc 4861 cagcgtatgg tggaacaggc ggctcgagaa aaaactgaaa agatcatcag tgcagaactc 4921 caaaaagcag ctgccaatcc agacttacag ggacacttac ctagtttcca gcagtctgct 4981 tttaaccggg tggtgagcag tgtgtcatct tctcctcgac aaacaatgga ctatgatgat 5041 gaagaaacag actctgatga agacattcga gagacatatg gctacgacat gaaggacaca 5101 gccagtgtga agtcctctag tagtcttgaa cccaatcttt tttgtgatga agagattccc 5161 atcaaatccg aggaggtggt gacccacatg tggacagcac cttcattttg tgcagagcat 5221 gcttattctt ctgcttctaa gagttgttct caaggttcta gcaccccaag gaaacaacct 5281 cggaagagcc ctttggtgcc ccgaagtttg gaacctccag tgctggagtt gtcacctgga 5341 gctaaggcac aactggaaga acttatgatg gttggagatc tcctggaagt atctctggac 5401 gagactcaac acatatggcg gattttgcag gccacacacc caccctctga agacagattc 5461 ttgcatatca tggaggatga cagcatggaa gagaaaccac taaaagtgaa aggaaaggac 5521 tcttcagaga agaaacggaa acggaagcta gaaaaggtag agcaactttt tggagaagga 5581 aaacagaagt ccaaggagtt aaagaaaatg gacaaaccta gaaagaagaa attaaaatta 5641 ggtgcagaca aatcaaagga gctgaataaa ctggccaaga aactagcaaa agaagaagag 5701 agaaagaaaa agaaggagaa ggctgctgca gccaaagttg aacttgtgaa agagagcact 5761 gaaaagaaaa gagagaaaaa ggtgctggac atcccctcaa agtatgactg gtcaggagca 5821 gaggagtctg atgatgagaa tgctgtgtgc gcagcacaga actgccaaag gccctgcaag 5881 gacaagggag ttgtatttgt aacggaagaa gagaagaata aaaaatatta gttttaaaag 5941 tggcctatgt gactgctttt ctaaaaaggt agactgggta caatgtgatg gtggctgtga 6001 tgagtggttt catcaagttt gtgtgggtgt atctccagaa atggctgaaa atgaagatta 6061 catctgtata aactgtgcaa agaagcaggg gccagttagc ccaggtccag caccacctcc 6121 ttccttcata atgagctaca aactaccaat ggaggatctt aaagagacca gttagcagat 6181 gcttggttag tttgggacat ggggggacat ggaccacatt gagaccttag tcatcaagta 6241 gagtggttta tatccacttg gaatgttgct tccaaagatg aatggccttc agagaaagtc 6301 cccttagtgc tggcttcctc tttgcatgga ctctgtgggt tacattctct atcaacatat 6361 ctatgcagag ggtgtcttct ttggtacaac agccaatatt tcatgtctcc tttgagtgtg 6421 gtttactgca ttaaggccag atgcttaatt gagctctagg gtggctggtt agtattaata 6481 cattggtgtg ctaacagggc atataggatg tggcttttgt ccagctgata gtagttagag 6541 gcttacaact taggagcagc accaactgaa ggtgctaatt gcttggatct ccttcattgg 6601 gatagttgga gagggattgg agtaccactt tccttccact gttacctggt acttaatgcc 6661 ctaaagatac aactaggagt aacagggcca aagttatttc tgttagacgt caaggaatgg 6721 tatcacagtc tattgacctc agcgatttgt gcttgtttgt gctagaagaa catcccaaat 6781 aggagaacct ctcacaagct ggggcaggtc accttatctt tgtaagatga ggatatcatc 6841 tagatcagaa atctgactag attggattct gaggagaaga acctactaca aggcaaggag 6901 ccgttttttg gctttgaaaa gtcttgctgt cttgggtcta cattttaggg aagagcaggt 6961 acatggatcc aggcttctgc caaaaaaaaa aagagaagaa gatgacgatg atgaccagtc 7021 gtactatctt actgagccac agtgatgcat gcttttcggg gaaaacttca ttcacaagta 7081 ttccagacac caggcttcag gcatggccat gagcaagacc agcaaataac agctttttgc 7141 cttgcagccc tgaccccaat gtctgctgtt tccaacactg gtgatttcta actacggccc 7201 acagcagatg ctgttgaata acaccatggc ttcatcagag gatgtggggt tgtagtacct 7261 ctgggtgatg aagttgtttt agtaaatcca tttttaaaaa aaaaaaaaaa tctagagggc 7321 cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct 7381 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc 7441 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt 7501 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat 7561 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg 7621 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 7681 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 7741 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 7801 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 7861 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 7921 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 7981 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 8041 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg 8101 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 8161 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 8221 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 8281 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 8341 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 8401 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag 8461 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt 8521 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg 8581 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg 8641 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg 8701 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg 8761 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca 8821 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc 8881 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc 8941 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca 9001 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga 9061 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg 9121 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg 9181 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg 9241 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga 9301 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag 9361 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct 9421 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata 9481 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 9541 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag 9601 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 9661 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 9721 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 9781 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 9841 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 9901 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 9961 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 10021 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 10081 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 10141 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 10201 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 10261 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 10321 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 10381 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 10441 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 10501 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 10561 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 10621 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 10681 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 10741 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 10801 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 10861 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 10921 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 10981 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 11041 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 11101 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 11161 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 11221 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 11281 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 11341 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 11401 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 11461 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 11521 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 11581 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 11641 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 11701 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 11761 gccacctgac gtc //
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