pcDNA3/HA-FLAG RBP2 H483A

价格:1500元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

买家导航

载体基本信息

质粒名称: pcDNA3/HA-FLAG RBP2 H483A 
出品公司: Addgene
目录编号: 14801
存储实验室: William Kaelin

载体骨架基本信息
载体名称: pcDNA3
载体出品公司: --
载体抗性: 氨苄青霉素
空载体大小: 5446 bp
完整质粒大小: --
载体修饰: --
载体类型: 哺乳细胞表达
筛选标记: 新霉素G418
高拷贝/低拷贝: 低拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: Retinoblastoma Binding Protein 2,RBP2,JARID1A,KDM5A
物种来源: H. sapiens (human)
插入大小: 4900 bp
基因突变: Changed His 483 to Ala, R46W
GenBank编号 --
启动子 --
标签/融合蛋白:
HA(骨干网上的N个终端)
标志(主干上的N个终端)
克隆方法: 限制性内切酶
5' 克隆位点: --
3' 克隆位点: --
5' 测序引物 pCAAT
3' 测序引物 --

文献引用方法
材料和方法部分: pcDNA3/HA-FLAG RBP2 H483A was a gift from William Kaelin (Addgene plasmid # 14801 ; http://n2t.net/addgene:14801 ; RRID:Addgene_14801)
参考文献部分: The retinoblastoma binding protein RBP2 is an H3K4 demethylase. Klose RJ, Yan Q, Tothova Z, Yamane K, Erdjument-Bromage H, Tempst P, Gilliland DG, Zhang Y, Kaelin WG. Cell. 2007 Mar 9. 128(5):889-900. 10.1016/j.cell.2007.02.013 PubMed 17320163

载体图谱质粒图谱和多克隆位点信息

pcDNA3-HA-FLAG RBP2 H483A质粒图谱

载体简介

载体序列

LOCUS       pcDNA3/HA-FLAG RBP2 H483A              11773 bp ds-DNA     circular SYN 29-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3/HA-FLAG RBP2 H483A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11773)
  AUTHORS   Klose RJ, Yan Q, Tothova Z, Yamane K, Erdjument-Bromage H, Tempst P,
            Gilliland DG, Zhang Y, Kaelin WG
  TITLE     The retinoblastoma binding protein RBP2 is an H3K4 demethylase.
  JOURNAL   Cell. 2007 Mar 9. 128(5):889-900.
  PUBMED    17320163
REFERENCE   2  (bases 1 to 11773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 29, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11773
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             907..933
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             952..975
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(7326..7344)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7327..7344)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7364..7381)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7370..7594
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7640..8068
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7727..7746)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7937..7958
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8077..8097)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8082..8411
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8262..8397
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8324..8343
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8478..9272
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8532..8551)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9142..9161
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9446..9567
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9483..9502)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9537..9556
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9616..9632)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9616..9632)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9629..9651)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9640..9656
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9664..9694)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9709..9730
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9847..9864)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10018..10606)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10098..10117)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10777..11637)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11400..11419
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11638..11742)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 atggcgtacc catacgatgt tccagattac gccaggatcc atcgaggagg agactacaag
      961 gacgacgatg acaagggagg acgatgtcga cgaattctgc agatggggat ccgtcgacgg
     1021 atggcgggcg tggggccggg gggctacgcg gcggagttcg tgccaccgcc agagtgcccc
     1081 gtctttgagc cgagttggga ggagttcaca gatccgctca gctttatcgg ccgcatctgg
     1141 cctttggcgg agaaaaccgg catctgcaaa attcggccgc ccaaggactg gcagcctcca
     1201 tttgcctgtg aagtaaaaag ctttcgtttc actccaagag tccagcgcct gaatgaactt
     1261 gaggcaatga ccagagtgag attggatttc ttggatcaac tagcaaaatt ttgggaactt
     1321 caaggatcta ctctgaagat ccctgtggta gagagaaaaa tcctggatct gtatgctttg
     1381 agcaagattg ttgccagcaa aggaggtttt gaaatggtca ccaaagagaa gaaatggtct
     1441 aaagtgggta gtcgcttggg atatctgcca ggaaaaggaa ctgggtctct tttgaagtca
     1501 cattatgaaa gaattctcta cccatatgag cttttccagt ctggtgtgag ccttatgggt
     1561 gtgcagatgc ctaatttaga tcttaaagaa aaagtggagc ctgaggttct tagcactgat
     1621 acccaaactt ccccagagcc aggcacaagg atgaacattc tgccgaagag aacaagacgt
     1681 gtgaagactc agtcagaatc tggagatgtg agtagaaaca cggaactgaa gaaacttcag
     1741 atttttgggg ctgggcccaa ggttgtgggc ttggcaatgg gaacaaaaga taaagaagat
     1801 gaggtcaccc gaagacgaaa agttaccaac aggtcagacg catttaacat gcaaatgaga
     1861 caacggaaag gcactctctc tgttaacttt gttgatctct atgtttgtat gttttgtggt
     1921 cggggaaaca atgaagataa attgcttttg tgtgatggat gtgatgacag ctatcataca
     1981 ttttgtctaa ttcctccact acctgatgtg cccaaaggag actggaggtg tcctaaatgt
     2041 gtcgccgagg aatgtagcaa acctcgagaa gcctttggat ttgaacaagc tgtacgagag
     2101 tatacacttc agagctttgg agagatggca gataatttta agtctgatta ttttaatatg
     2161 ccagtccata tggttcccac agaactagta gaaaaggaat tttggcggct ggtaagcagc
     2221 attgaagaag atgttattgt ggaatatgga gcagatatct cctcaaaaga ctttggaagt
     2281 ggatttccgg tgaaggatgg gcggagaaag attctgccag aagaagagga atatgcactt
     2341 tctggttgga atttgaataa catgcctgtc ctggaacagt ctgttcttgc acatattaat
     2401 gtggacatct ctggtatgaa agtgccgtgg ctctatgtgg gaatgtgctt ctcttctttt
     2461 tgctgggcca ttgaggatca ctggagttat tccatcaact acttgcactg gggggagcca
     2521 aagacatggt atggtgtgcc atctcatgct gcagagcaac tggaggaggt gatgagagag
     2581 ctggcccccg agttatttga atcccagcct gatcttctgc atcagttagt taccatcatg
     2641 aaccccaacg tgctaatgga gcatggtgtg cctgtgtaca ggaccaatca gtgtgctggc
     2701 gagtttgttg tgacatttcc tcgtgcctat cactctggat ttaaccaggg ctacaacttt
     2761 gctgaagctg tgaacttctg tactgctgac tggttgccca ttggacgtca atgtgtaaat
     2821 cattaccgac gcctaaggcg ccactgtgtc ttttcacacg aggaactaat tttcaagatg
     2881 gcagcagatc cagaatgctt agatgtgggg ctggctgcca tggtctgcaa agaattgact
     2941 ctcatgactg aagaagaaac acgattaaga gagtctgttg tacagatggg tgtcctgatg
     3001 tcagaagaag aagtgtttga acttgttcct gatgatgagc ggcagtgttc agcatgcaga
     3061 accacatgtt ttctctctgc tctcacatgt tcctgtaatc ctgagcggct tgtatgtctc
     3121 taccatccaa ctgatctgtg cccctgcccc atgcagaaga aatgtcttag atatcgctac
     3181 ccattagaag acctcccttc tctgctatat ggtgtaaaag tcagggcaca gtcctatgac
     3241 acttgggtca gtcgtgttac agaagcattg tctgctaact tcaaccacaa aaaagatttg
     3301 attgaattgc gagtaatgct ggaagatgct gaggatagga aatacccaga gaatgatctc
     3361 tttcgaaaac tcagggatgc tgtaaaagaa gctgagacct gtgcttctgt ggctcagctg
     3421 cttctgagca aaaagcagaa acacagacag agcccagata gtgggaggac tcggaccaaa
     3481 ctgacagtgg aagaattgaa ggcctttgtc caacaacttt ttagtcttcc gtgtgtcatc
     3541 agccaagctc ggcaagtaaa gaatctgcta gatgatgtgg aagagtttca tgaacgtgct
     3601 caggaggcca tgatggatga aaccccagat tcttccaaac tccagatgtt gatagatatg
     3661 ggctctagtc tctatgtgga actccctgaa ttaccacgac tgaagcaaga gctacaacag
     3721 gctcggtggt tggacgaagt aagactgacc ttatcagatc cgcaacaagt cactttggat
     3781 gtcatgaaga agctgataga ctctggggta gggttggcac cccaccatgc tgtggagaaa
     3841 gcaatggctg aactacagga gctccttaca gtctctgaac gatgggaaga aaaggctaag
     3901 gtctgcctac aggcaagacc gaggcacagt gtggcaagtt tagaaagcat tgtgaatgaa
     3961 gccaagaaca ttccagcctt tctacccaat gtgttgtcct tgaaagaagc cttacaaaag
     4021 gctcgagaat ggaccgctaa agtggaagct attcagagtg gcagcaatta cgcttatttg
     4081 gagcagcttg agagcttgtc tgcgaaagga cgccctattc ctgtgcgtct tgaagcactg
     4141 ccgcaagtgg aatcacaggt agcagcagca cgggcatgga gagaacggac tgggcggacg
     4201 tttcttaaga agaattctag ccatacattg ttacaggtgc tgagcccccg gaccgacatt
     4261 ggtgtatatg ggagtggcaa aaataggagg aaaaaagtaa aagaactaat agaaaaagaa
     4321 aaagaaaagg atctggacct ggagcctctg agtgatctgg aggaaggatt ggaggaaacc
     4381 agagatacag ccatggtggt ggcagttttc aaagaacggg agcaaaaaga gattgaagcc
     4441 atgcattctc tcagagcagc caacctagcc aagatgacaa tggtggaccg catagaagaa
     4501 gtaaaatttt gcatttgccg caagacagcc agtgggttta tgctacagtg tgagctctgc
     4561 aaagactggt tccataacag ctgtgttcct cttcctaaat caagttccca aaaaaaagga
     4621 tccagctggc aagctaaaga agtaaaattc ctttgccctc tttgtatgcg gtctcgaagg
     4681 cccaggctag agactattct gtcactcctg gtatcccttc agaagttgcc cgtacggttg
     4741 cctgaaggag aggccctgca gtgtttgaca gaacgtgcta tgagttggca agatagagcg
     4801 cggcaggctc tagccacaga tgaactatcc tctgccctgg ccaaactatc tgtgttgagc
     4861 cagcgtatgg tggaacaggc ggctcgagaa aaaactgaaa agatcatcag tgcagaactc
     4921 caaaaagcag ctgccaatcc agacttacag ggacacttac ctagtttcca gcagtctgct
     4981 tttaaccggg tggtgagcag tgtgtcatct tctcctcgac aaacaatgga ctatgatgat
     5041 gaagaaacag actctgatga agacattcga gagacatatg gctacgacat gaaggacaca
     5101 gccagtgtga agtcctctag tagtcttgaa cccaatcttt tttgtgatga agagattccc
     5161 atcaaatccg aggaggtggt gacccacatg tggacagcac cttcattttg tgcagagcat
     5221 gcttattctt ctgcttctaa gagttgttct caaggttcta gcaccccaag gaaacaacct
     5281 cggaagagcc ctttggtgcc ccgaagtttg gaacctccag tgctggagtt gtcacctgga
     5341 gctaaggcac aactggaaga acttatgatg gttggagatc tcctggaagt atctctggac
     5401 gagactcaac acatatggcg gattttgcag gccacacacc caccctctga agacagattc
     5461 ttgcatatca tggaggatga cagcatggaa gagaaaccac taaaagtgaa aggaaaggac
     5521 tcttcagaga agaaacggaa acggaagcta gaaaaggtag agcaactttt tggagaagga
     5581 aaacagaagt ccaaggagtt aaagaaaatg gacaaaccta gaaagaagaa attaaaatta
     5641 ggtgcagaca aatcaaagga gctgaataaa ctggccaaga aactagcaaa agaagaagag
     5701 agaaagaaaa agaaggagaa ggctgctgca gccaaagttg aacttgtgaa agagagcact
     5761 gaaaagaaaa gagagaaaaa ggtgctggac atcccctcaa agtatgactg gtcaggagca
     5821 gaggagtctg atgatgagaa tgctgtgtgc gcagcacaga actgccaaag gccctgcaag
     5881 gacaagggag ttgtatttgt aacggaagaa gagaagaata aaaaatatta gttttaaaag
     5941 tggcctatgt gactgctttt ctaaaaaggt agactgggta caatgtgatg gtggctgtga
     6001 tgagtggttt catcaagttt gtgtgggtgt atctccagaa atggctgaaa atgaagatta
     6061 catctgtata aactgtgcaa agaagcaggg gccagttagc ccaggtccag caccacctcc
     6121 ttccttcata atgagctaca aactaccaat ggaggatctt aaagagacca gttagcagat
     6181 gcttggttag tttgggacat ggggggacat ggaccacatt gagaccttag tcatcaagta
     6241 gagtggttta tatccacttg gaatgttgct tccaaagatg aatggccttc agagaaagtc
     6301 cccttagtgc tggcttcctc tttgcatgga ctctgtgggt tacattctct atcaacatat
     6361 ctatgcagag ggtgtcttct ttggtacaac agccaatatt tcatgtctcc tttgagtgtg
     6421 gtttactgca ttaaggccag atgcttaatt gagctctagg gtggctggtt agtattaata
     6481 cattggtgtg ctaacagggc atataggatg tggcttttgt ccagctgata gtagttagag
     6541 gcttacaact taggagcagc accaactgaa ggtgctaatt gcttggatct ccttcattgg
     6601 gatagttgga gagggattgg agtaccactt tccttccact gttacctggt acttaatgcc
     6661 ctaaagatac aactaggagt aacagggcca aagttatttc tgttagacgt caaggaatgg
     6721 tatcacagtc tattgacctc agcgatttgt gcttgtttgt gctagaagaa catcccaaat
     6781 aggagaacct ctcacaagct ggggcaggtc accttatctt tgtaagatga ggatatcatc
     6841 tagatcagaa atctgactag attggattct gaggagaaga acctactaca aggcaaggag
     6901 ccgttttttg gctttgaaaa gtcttgctgt cttgggtcta cattttaggg aagagcaggt
     6961 acatggatcc aggcttctgc caaaaaaaaa aagagaagaa gatgacgatg atgaccagtc
     7021 gtactatctt actgagccac agtgatgcat gcttttcggg gaaaacttca ttcacaagta
     7081 ttccagacac caggcttcag gcatggccat gagcaagacc agcaaataac agctttttgc
     7141 cttgcagccc tgaccccaat gtctgctgtt tccaacactg gtgatttcta actacggccc
     7201 acagcagatg ctgttgaata acaccatggc ttcatcagag gatgtggggt tgtagtacct
     7261 ctgggtgatg aagttgtttt agtaaatcca tttttaaaaa aaaaaaaaaa tctagagggc
     7321 cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct
     7381 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     7441 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     7501 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     7561 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     7621 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     7681 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     7741 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     7801 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     7861 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     7921 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     7981 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     8041 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     8101 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     8161 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     8221 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     8281 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     8341 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     8401 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     8461 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     8521 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     8581 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     8641 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     8701 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     8761 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     8821 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     8881 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     8941 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     9001 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     9061 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     9121 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     9181 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     9241 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     9301 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     9361 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     9421 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     9481 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     9541 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     9601 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     9661 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     9721 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     9781 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     9841 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     9901 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     9961 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    10021 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    10081 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    10141 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    10201 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    10261 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    10321 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    10381 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    10441 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    10501 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    10561 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    10621 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    10681 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    10741 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    10801 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    10861 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    10921 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    10981 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    11041 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    11101 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    11161 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    11221 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    11281 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    11341 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    11401 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    11461 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    11521 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    11581 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    11641 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    11701 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    11761 gccacctgac gtc
//

载体图谱质粒图谱pdf版和相关资料下载

载体质粒应用举例

分享到:

全部载体分类

相关载体

相关文章

相关问答

Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng 沪ICP备2021037978号-1
欢迎您的访问和咨询....